Service Centre category(ies)
Cell Analysis / Flow Cytometry
Description of the Service Centre
The Flow Cytometry Unit provides technical support on flow cytometry. Currently, it is equipped with a Becton Dickinson FACSCalibur cell analyser and a Partec CyFlow Space cell sorter. The cytometry unit provides scientific and technical support for those wishing to use flow cytometry in their studies and is available to all CNC researchers, as well for those external to the CNC, after prior arrangement.
Service Centre web address
http://www.cnbc.pt/services/flow_cytometry_2.asp
Contact person's name
Isabel Nunes Correia
Contact person's e-mail
inunes@cnc.uc.pt
Contact person's telephone
+351 239 820 190
Service
Cell Sorting
Available for external use?
Yes
Comments
Analysis and sort of different cell populations.
Service
Cell Analysis
Available for external use?
Yes
Comments
Analysis of cells based on size, granularity and whether the cell is carrying fluorescent molecules in the form of either antibodies or dyes.
Equipment
Partec CyFlow Space
Service type: self-service or full-service?
Full-service
Comments
The equipment contains the 405 nm,488 nm and 635 nm lasers and seven channels for fluorescence detection. Based on the method of true volumetric absolute counting it is possible to analyze sample concentrations. The Partec particle and cell sorter serves as an optimized module for simplified cell sorting, possessing a sorting device that can be sterilized safely. For further details on the equipment, please visit the webpage http://www.cnbc.pt/services/flow_cytometry_2.asp.
Equipment
Becton Dickinson FACSCalibur
Service type: self-service or full-service?
Full-service
Comments
The FACSCalibur contains the 488 nm and 635 nm lasers. Three wavelengths are detected from the 488 nm laser, 530/30 nm, 585/42 nm and >670 nm. One wavelength is detected from the 635 nm laser, 661/16 nm. For further details on the equipment, please visit the webpage http://www.cnbc.pt/services/flow_cytometry_2.asp.