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Service Centre

Flow Cytometry Unit

Center for Neuroscience and Cell Biology

Service Centre category(ies)

Cell Analysis / Flow Cytometry

Description of the Service Centre

The Flow Cytometry Unit provides technical support on flow cytometry. Currently, it is equipped with a Becton Dickinson FACSCalibur cell analyser and a Partec CyFlow Space cell sorter. The cytometry unit provides scientific and technical support for those wishing to use flow cytometry in their studies and is available to all CNC researchers, as well for those external to the CNC, after prior arrangement.

Service Centre web address

http://www.cnbc.pt/services/flow_cytometry_2.asp

Contact person's name

Isabel Nunes Correia

Contact person's e-mail

inunes@cnc.uc.pt

Contact person's telephone

+351 239 820 190

Services

Service

Cell Sorting

Available for external use?

Yes

Comments

Analysis and sort of different cell populations.

Service

Cell Analysis

Available for external use?

Yes

Comments

Analysis of cells based on size, granularity and whether the cell is carrying fluorescent molecules in the form of either antibodies or dyes.

Equipments

Equipment

Partec CyFlow Space

Service type: self-service or full-service?

Full-service

Comments

The equipment contains the 405 nm,488 nm and 635 nm lasers and seven channels for fluorescence detection. Based on the method of true volumetric absolute counting it is possible to analyze sample concentrations. The Partec particle and cell sorter serves as an optimized module for simplified cell sorting, possessing a sorting device that can be sterilized safely. For further details on the equipment, please visit the webpage http://www.cnbc.pt/services/flow_cytometry_2.asp.

Equipment

Becton Dickinson FACSCalibur

Service type: self-service or full-service?

Full-service

Comments

The FACSCalibur contains the 488 nm and 635 nm lasers. Three wavelengths are detected from the 488 nm laser, 530/30 nm, 585/42 nm and >670 nm. One wavelength is detected from the 635 nm laser, 661/16 nm. For further details on the equipment, please visit the webpage http://www.cnbc.pt/services/flow_cytometry_2.asp.

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