Service Centre category(ies)
Description of the Service Centre
The Laboratory of Support to Research in Molecular Medicine was created to promote the development of medical research. It offers logistical, operational and consulting services in the context of biomedical research projects, as well as custom molecular biology laboratory services, mutant and transgenic mouse models production, and scientific training.
Service Centre web address
https://laimm.med.up.pt/index.php/en/
Contact person's name
Miguel Soares
Contact person's e-mail
mlsoares@med.up.pt
Contact person's telephone
220426779
Last edited on
2017-03-15 09:48:53
Service
Support to Scientific Research: assistance in experimental execution
Available for external use?
Yes
Comments
LAIMM supports the experimental execution of any research projects that may fall within its scientific scope and are deemed feasible, regardless of having or not been involved in the conception/design of these projects.
Service
Support to Scientific Research: assistance in project design
Available for external use?
Yes
Comments
This option is aimed at those groups which have biological material and/or relevant clinical data but due to a lack of available time or experience in laboratory research require support in conceiving and designing a research project.
Service
Laboratory Service: Molecular Biology - Genomics
Available for external use?
Yes
Comments
Gene expression studies. Molecular cloning (recombinant DNA techniques). DNA-protein interactions. Genotyping. Molecular Typing
Service
Laboratory Services: Molecular Biology - Proteomics
Available for external use?
Yes
Comments
Protein expression studies. Protein-protein interactions. Protein separation by two-dimensional electrophoresis. Tagged protein purification (including liquid chromatography)
Service
Laboratory Service: Production of Mutant Animals / Genetic manipulation
Available for external use?
Yes
Comments
Genetic manipulation of the mouse (knock-out, knock-in, deletion). CRISPR/Cas9 and homologous recombination – mediated mutagenesis. Pronuclear Injection. ES cell Injection. Mouse Line Rederivation. Embryo Cryopreservation. Sperm Cryopreservation. In-Vitro Fertilization. Vasectomy and other Surgical Procedures.
Service
Laboratory Service: Microscopy/Imaging
Available for external use?
Yes
Comments
1-Bright field microscopy with image aquisition. 2-Fluorescence Microscopy: live cell / embryo imaging; time-lapse; ApoTome optical sectioning; confocal microscopy.
Equipment
Sistema de focagem isoeléctrica de proteínas
Service type: self-service or full-service?
Full-service
Comments
Bio-Rad Protean i12 IEF Cell; Necessária sessão tutorial inicial para se tornar utilizador acreditado
Equipment
Sistema de transferência de proteínas
Service type: self-service or full-service?
Self-service
Comments
Bio-Rad Trans-Blot Turbo system. Necessária sessão tutorial inicial para se tornar utilizador acreditado
Equipment
Sistema de cromatografia de baixa pressão com colector de amostras
Service type: self-service or full-service?
Full-service
Comments
Bio-Rad Biologic LP system + BioFrac Fraction collector. Necessária sessão tutorial inicial para se tornar utilizador acreditado
Equipment
Sistema aquisição imagens de fluorescência (Phosphorimager)
Service type: self-service or full-service?
Self-service
Comments
Molecular Dynamics Storm 860
Equipment
Sistema de filtração de água
Service type: self-service or full-service?
Self-service
Comments
MilliPore Milli-Q Integral 3
Equipment
Espectrofotómetro para quantificação de microvolumes de amostra
Service type: self-service or full-service?
Self-service
Comments
Ácidos nucleicos/proteína. Thermo Scientific NanoDrop 2000
Equipment
Bioanalizador - plataforma para análise de ácidos nucleicos
Service type: self-service or full-service?
Full-service
Comments
Agilent Byoanalizer 2100. Integridade e quantificação de ácidos nucleicos. Necessária sessão tutorial inicial para se tornar utilizador acreditado
Equipment
Sistema de PCR em tempo real
Service type: self-service or full-service?
Self-service
Comments
Applied Biosystems Real-Time PCR StepOne Plus. Necessária sessão tutorial inicial para se tornar utilizador acreditado
Equipment
Termociclador com gradiente
Service type: self-service or full-service?
Self-service
Comments
Bio-Rad T100 Thermal. Necessária sessão tutorial inicial para se tornar utilizador acreditado
Equipment
Homogeneizador de tecidos
Service type: self-service or full-service?
Self-service
Comments
Polytron PT2500E.
Equipment
Concentrador de amostras com bomba de vácuo
Service type: self-service or full-service?
Self-service
Comments
Thermo Scientific Savant SPD121P-230V SpeedVac concentrator. Necessária sessão tutorial inicial para se tornar utilizador acreditado
Equipment
Criostato/micrótomo em câmara refrigerada
Service type: self-service or full-service?
Self-service
Comments
Thermo Micron HM560. Necessária sessão tutorial inicial para se tornar utilizador acreditado.
Equipment
Homogeneizador automático de tecidos multi-amostra
Service type: self-service or full-service?
Self-service
Comments
Roche MagNA lyser. Necessária sessão tutorial inicial para se tornar utilizador acreditado
Equipment
Forja de agulhas / capilares de vidro
Service type: self-service or full-service?
Full-service
Comments
Sutter P-97 Micropipette Puller
Equipment
Sistema aquisição imagens UV, quimioluminiscência e fluorescência
Service type: self-service or full-service?
Self-service
Comments
Bio-Rad ChemiDoc MP.
Equipment
Micromanipulação de embriões
Service type: self-service or full-service?
Full-service
Comments
Leica AM6000 automated motorized inverted microscope + TransferMan NK2 Automated Micromanipulators and Microinjectors.