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Service Centre

Laboratory of Support to Research in Molecular Medicine (LAIMM)

Faculdade de Medicina da Universidade do Porto

Service Centre category(ies)

Genomics / Gene Expression Analysis / DNA Sequencing / DNA Synthesis / Real-Time PCR / Genotyping / Microarray

Description of the Service Centre

The Laboratory of Support to Research in Molecular Medicine was created to promote the development of medical research. It offers logistical, operational and consulting services in the context of biomedical research projects, as well as custom molecular biology laboratory services, mutant and transgenic mouse models production, and scientific training.

Service Centre web address

https://laimm.med.up.pt/index.php/en/

Contact person's name

Miguel Soares

Contact person's e-mail

mlsoares@med.up.pt

Contact person's telephone

220426779

Last edited on

2017-03-15 09:48:53

Services

Service

Support to Scientific Research: assistance in experimental execution

Available for external use?

Yes

Comments

LAIMM supports the experimental execution of any research projects that may fall within its scientific scope and are deemed feasible, regardless of having or not been involved in the conception/design of these projects.

Service

Support to Scientific Research: assistance in project design

Available for external use?

Yes

Comments

This option is aimed at those groups which have biological material and/or relevant clinical data but due to a lack of available time or experience in laboratory research require support in conceiving and designing a research project.

Service

Laboratory Service: Molecular Biology - Genomics

Available for external use?

Yes

Comments

Gene expression studies. Molecular cloning (recombinant DNA techniques). DNA-protein interactions. Genotyping. Molecular Typing

Service

Laboratory Services: Molecular Biology - Proteomics

Available for external use?

Yes

Comments

Protein expression studies. Protein-protein interactions. Protein separation by two-dimensional electrophoresis. Tagged protein purification (including liquid chromatography)

Service

Laboratory Service: Production of Mutant Animals / Genetic manipulation

Available for external use?

Yes

Comments

Genetic manipulation of the mouse (knock-out, knock-in, deletion). CRISPR/Cas9 and homologous recombination – mediated mutagenesis. Pronuclear Injection. ES cell Injection. Mouse Line Rederivation. Embryo Cryopreservation. Sperm Cryopreservation. In-Vitro Fertilization. Vasectomy and other Surgical Procedures.

Service

Laboratory Service: Microscopy/Imaging

Available for external use?

Yes

Comments

1-Bright field microscopy with image aquisition. 2-Fluorescence Microscopy: live cell / embryo imaging; time-lapse; ApoTome optical sectioning; confocal microscopy.

Equipments

Equipment

Sistema de focagem isoeléctrica de proteínas

Service type: self-service or full-service?

Full-service

Comments

Bio-Rad Protean i12 IEF Cell; Necessária sessão tutorial inicial para se tornar utilizador acreditado

Equipment

Sistema de transferência de proteínas

Service type: self-service or full-service?

Self-service

Comments

Bio-Rad Trans-Blot Turbo system. Necessária sessão tutorial inicial para se tornar utilizador acreditado

Equipment

Sistema de cromatografia de baixa pressão com colector de amostras

Service type: self-service or full-service?

Full-service

Comments

Bio-Rad Biologic LP system + BioFrac Fraction collector. Necessária sessão tutorial inicial para se tornar utilizador acreditado

Equipment

Sistema aquisição imagens de fluorescência (Phosphorimager)

Service type: self-service or full-service?

Self-service

Comments

Molecular Dynamics Storm 860

Equipment

Sistema de filtração de água

Service type: self-service or full-service?

Self-service

Comments

MilliPore Milli-Q Integral 3

Equipment

Espectrofotómetro para quantificação de microvolumes de amostra

Service type: self-service or full-service?

Self-service

Comments

Ácidos nucleicos/proteína. Thermo Scientific NanoDrop 2000

Equipment

Bioanalizador - plataforma para análise de ácidos nucleicos

Service type: self-service or full-service?

Full-service

Comments

Agilent Byoanalizer 2100. Integridade e quantificação de ácidos nucleicos. Necessária sessão tutorial inicial para se tornar utilizador acreditado

Equipment

Sistema de PCR em tempo real

Service type: self-service or full-service?

Self-service

Comments

Applied Biosystems Real-Time PCR StepOne Plus. Necessária sessão tutorial inicial para se tornar utilizador acreditado

Equipment

Termociclador com gradiente

Service type: self-service or full-service?

Self-service

Comments

Bio-Rad T100 Thermal. Necessária sessão tutorial inicial para se tornar utilizador acreditado

Equipment

Homogeneizador de tecidos

Service type: self-service or full-service?

Self-service

Comments

Polytron PT2500E.

Equipment

Concentrador de amostras com bomba de vácuo

Service type: self-service or full-service?

Self-service

Comments

Thermo Scientific Savant SPD121P-230V SpeedVac concentrator. Necessária sessão tutorial inicial para se tornar utilizador acreditado

Equipment

Criostato/micrótomo em câmara refrigerada

Service type: self-service or full-service?

Self-service

Comments

Thermo Micron HM560. Necessária sessão tutorial inicial para se tornar utilizador acreditado.

Equipment

Homogeneizador automático de tecidos multi-amostra

Service type: self-service or full-service?

Self-service

Comments

Roche MagNA lyser. Necessária sessão tutorial inicial para se tornar utilizador acreditado

Equipment

Forja de agulhas / capilares de vidro

Service type: self-service or full-service?

Full-service

Comments

Sutter P-97 Micropipette Puller

Equipment

Sistema aquisição imagens UV, quimioluminiscência e fluorescência

Service type: self-service or full-service?

Self-service

Comments

Bio-Rad ChemiDoc MP.

Equipment

Micromanipulação de embriões

Service type: self-service or full-service?

Full-service

Comments

Leica AM6000 automated motorized inverted microscope + TransferMan NK2 Automated Micromanipulators and Microinjectors.

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