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Service Centre

Advanced Flow Cytometry Unit

IBMC - Institute for Molecular and Cell Biology

Service Centre category(ies)

Cell Analysis / Flow Cytometry

Description of the Service Centre

The AFCU provides investigators with equipment for acquisition and analysis of flow cytometric data and for cell sorting from single cell suspension using fluorescence. Furthermore, the AFCU gives training and consulting for researchers that intend to use flow cytometry in their projects; and helps analyzing and interpreting data. A number of applications, including the multicolour analysis of cell phenotype, gene expression, membrane potential, cell cycle, among others, can be performed.

Service Centre web address

http://www.ibmc.up.pt/research/research-facilities/advanced-flow-cytometry-unit

Contact person's name

Catarina Leitão

Contact person's e-mail

catarina.leitao@ibmc.up.pt

Contact person's telephone

+351 226 074 900

Services

Service

cell sorting

Available for external use?

Yes

Comments

equipment: FACS Aria I - 2 lasers (488nm and 633nm) and allows the simultaneous analysis up to 9 parameters.

Service

Cell analysis

Available for external use?

Yes

Comments

equipment: FACS Calibur - 2 laser (488nm and 633nm) flow cytometer. FACS Calibur can measure up to 6 parameters (FSC, SSC and 4 fluorescence parameters) FACS Canto II - 3 laser flow cytometer (405nm, 488nm and 633nm). FACS Canto II can measure up to

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