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Service Centre

Advanced Light Microscopy

IBMC - Institute for Molecular and Cell Biology

Service Centre category(ies)

Microscopy

Description of the Service Centre

The Advance Light Microscopy (ALM) is an open access scientific core facility of IBMC dedicated to state-of the-art optical microscopy applications for biosciences. Multidimensional (6D) imaging of cells and tissues, high speed live cell microscopy, molecular analysis techniques (FRAP, FRET) and in vivo microscopy are some of the applications available to researchers

Service Centre web address

http://www.ibmc.up.pt/research/research-facilities/advanced-light-microscopy

Contact person's name

Paula Sampaio

Contact person's e-mail

sampaio@ibmc.up.pt

Contact person's telephone

226074900

Services

Service

Advanced Light Microscopy

Available for external use?

Yes

Comments

The ALM provides open access to advanced light microscopy systems as fluorescence widefield and laser scanning confocal microscopes, training in equipment use, scientific advisement in experiment planning, advanced formation, consulting and collaboration

Equipments

Equipment

Live Cell Imaging Workstation

Service type: self-service or full-service?

Self-service

Comments

Live Cell imaging workstation based in Zeiss Axiovert 200M widefield full motorized inverted microscope. Environmental control (temperature and CO2) for long time cell imaging. Motorized XY stage for multi-position acquisition.

Equipment

Laser Scanning Confocal Systems

Service type: self-service or full-service?

Self-service

Comments

ALM provide access to the Laser Scanning Confocal microscopes Leica TCS SP5II and Leica TCS SP2 AOBS equipped with 4 lasers, (405, 458, 476, 488, 496, 514, 561, 633 nm), Spectral detection, 3 detectors PMT (SP2) or 3 PMTs plus one HyD (SP5) , Tandem scanner (High-speed resonant plus conventional) (SP5), Motorized XY stage (SP5), Environmental chamber with temperature control and DIC

Equipment

Spinning Disk Confocal System

Service type: self-service or full-service?

Self-service

Comments

Live cell imaging workstation based in a Spinning Disk Andor Revolution XD equipped with 2 lasers (488 and 561 nm), Motorized X-Y stage, Fast Z stage, Environmental chamber with temperature and CO2 control and DIC

Equipment

3D Huygens Deconvolution & Analysis Software

Service type: self-service or full-service?

Self-service

Comments

Software suite for deconvolution, interactive analysis and volume visualization of 2D, 3D, multi-channel and time series images from fluorescence microscopes

Equipment

Widefield motorised research microscope

Service type: self-service or full-service?

Self-service

Comments

Widefield Zeiss AxioImager Z1 microscope for automatic multi-dimensional image acquisition in transmission (brightfield, phase contrast and DIC) and fluorescence techniques.

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