Service Centre category(ies)
Description of the Service Centre
The Advance Light Microscopy (ALM) is an open access scientific core facility of IBMC dedicated to state-of the-art optical microscopy applications for biosciences. Multidimensional (6D) imaging of cells and tissues, high speed live cell microscopy, molecular analysis techniques (FRAP, FRET) and in vivo microscopy are some of the applications available to researchers
Service Centre web address
http://www.ibmc.up.pt/research/research-facilities/advanced-light-microscopy
Contact person's name
Paula Sampaio
Contact person's e-mail
sampaio@ibmc.up.pt
Contact person's telephone
226074900
Service
Advanced Light Microscopy
Available for external use?
Yes
Comments
The ALM provides open access to advanced light microscopy systems as fluorescence widefield and laser scanning confocal microscopes, training in equipment use, scientific advisement in experiment planning, advanced formation, consulting and collaboration
Equipment
Live Cell Imaging Workstation
Service type: self-service or full-service?
Self-service
Comments
Live Cell imaging workstation based in Zeiss Axiovert 200M widefield full motorized inverted microscope. Environmental control (temperature and CO2) for long time cell imaging. Motorized XY stage for multi-position acquisition.
Equipment
Laser Scanning Confocal Systems
Service type: self-service or full-service?
Self-service
Comments
ALM provide access to the Laser Scanning Confocal microscopes Leica TCS SP5II and Leica TCS SP2 AOBS equipped with 4 lasers, (405, 458, 476, 488, 496, 514, 561, 633 nm), Spectral detection, 3 detectors PMT (SP2) or 3 PMTs plus one HyD (SP5) , Tandem scanner (High-speed resonant plus conventional) (SP5), Motorized XY stage (SP5), Environmental chamber with temperature control and DIC
Equipment
Spinning Disk Confocal System
Service type: self-service or full-service?
Self-service
Comments
Live cell imaging workstation based in a Spinning Disk Andor Revolution XD equipped with 2 lasers (488 and 561 nm), Motorized X-Y stage, Fast Z stage, Environmental chamber with temperature and CO2 control and DIC
Equipment
3D Huygens Deconvolution & Analysis Software
Service type: self-service or full-service?
Self-service
Comments
Software suite for deconvolution, interactive analysis and volume visualization of 2D, 3D, multi-channel and time series images from fluorescence microscopes
Equipment
Widefield motorised research microscope
Service type: self-service or full-service?
Self-service
Comments
Widefield Zeiss AxioImager Z1 microscope for automatic multi-dimensional image acquisition in transmission (brightfield, phase contrast and DIC) and fluorescence techniques.