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Service Centre

Cell Biology

iMed.ULisboa/ FFULisboa Research Institute for Medicines/ Faculdade de Farmácia, Universidade de Lisboa

Service Centre category(ies)

Cell Analysis / Flow Cytometry
Biobanks / Clinical Samples / Histology / Pathology
Microscopy
Molecular fractionation, isolation, purification and production
Genomics / Gene Expression Analysis / DNA Sequencing / DNA Synthesis / Real-Time PCR / Genotyping / Microarray
Proteomics / Peptide Synthesis

Description of the Service Centre

This facility is dedicated to the analysis of gene expression at DNA, RNA and protein level, routinely performing a multitude of biochemical and molecular biology techniques using standard equipment for electrophoresis, western blot, northern bot, as well absorbance plate reader, and gel, membrane and X-ray film imaging (Chemidoc MP and XRS; Bio-Rad).

Service Centre web address

http://imed.ulisboa.pt/research/resources-services/cell-biology-laboratory-2/

Contact person's name

Rui Castro

Contact person's e-mail

ruieduardocastro@ff.ulisboa.pt

Contact person's telephone

351217946400

Last edited on

2014-09-30 16:58:15

Services

Service

Cell Biology

Available for external use?

Yes

Comments

It is also equipped with end-point (Bio-Rad and Thermo) and real time PCR (Applied Biosystems 7300 Real-Time PCR System) thermocyclers, and a Guava easycyte 5HT benchtop flow cytometer for high-throughput cell analysis in 96-well format. Additionally, there is a stopped-flow (HiTech Scientific) and two Patch-clamp setups (Axon Instruments), used for kinetic analyses of ion channel activity in cells. Fluorescence and bright field microscopes (Zeiss) with dedicated cameras (Leica) and acquisition software are available.

Equipments

Equipment

Standard western and northern blot equipment (Bio-Rad)

Service type: self-service or full-service?

Full-service

Equipment

Chemidoc MP and XRS (Bio-Rad)

Service type: self-service or full-service?

Full-service

Equipment

Glomax multiplate reader (Promega)

Service type: self-service or full-service?

Full-service

Equipment

7300 Real-Time PCR System (Applied Biosystems)

Service type: self-service or full-service?

Full-service

Equipment

Termocycler Unocycler (VWR)

Service type: self-service or full-service?

Full-service

Equipment

Guava easycyte 5HT (Millipore)

Service type: self-service or full-service?

Full-service

Equipment

Stopped-flow (HiTech Scientific)

Service type: self-service or full-service?

Full-service

Equipment

Patch-clamp setups (Axon Instruments)

Service type: self-service or full-service?

Full-service

Equipment

Microscope Axio Scope.A1 coupled with a AxioCam HR (Zeiss)

Service type: self-service or full-service?

Full-service

Equipment

Microscope Axio Scope (Zeiss) + Leica DFC 490 (Leica)

Service type: self-service or full-service?

Full-service

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