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Service Centre

Cell Function

iMed.ULisboa/ FFULisboa Research Institute for Medicines/ Faculdade de Farmácia, Universidade de Lisboa

Service Centre category(ies)

Cell Analysis / Flow Cytometry
Biochemical / Chemical / Structural Analysis
Cell Culture and Manipulation
Microscopy
Molecular fractionation, isolation, purification and production
Stem Cells
Genomics / Gene Expression Analysis / DNA Sequencing / DNA Synthesis / Real-Time PCR / Genotyping / Microarray

Description of the Service Centre

The facility is tailored for biological evaluation of cell function, routinely determining cytotoxic and cytoprotective activities of synthetic and natural compounds, in multiple cell models, including immortalized and primary cultures and stem cells, with implemented capability of cell function assays up to 96 and 384-well format.

Service Centre web address

http://imed.ulisboa.pt/research/resources-services/cell-function-laboratory/

Contact person's name

Pedro Borralho

Contact person's e-mail

borralho@ff.ulisboa.pt

Contact person's telephone

351217946400

Last edited on

2014-10-01 14:49:46

Services

Service

Cell Biology Laboratory

Available for external use?

Yes

Comments

This service centre provides biological evaluation of cell function, routinely determining the role of transgenes and cytotoxic and cytoprotective activities of synthetic and natural extracts and compounds isolated from multiple sources (bacteria, plant, sponge, fungi, etc), in multiple cell models, including immortalized human, monkey, rat, and mouse cells, as well as in primary cultures of hepatocytes and brain cells from rat and mouse. Evaluation of celullar and subcellular molecular events is also carried out in close connection with the Cell Biology Laboratory.

Equipments

Equipment

Two dedicated cell culture rooms with 4 laminar flow cabinets

Service type: self-service or full-service?

Full-service

Equipment

4 CO2 incubators

Service type: self-service or full-service?

Full-service

Equipment

2 heated water baths

Service type: self-service or full-service?

Full-service

Equipment

Dedicated refrigerators and centrifuges

Service type: self-service or full-service?

Full-service

Equipment

Dedicated bright field inverted microscope (Zeiss)

Service type: self-service or full-service?

Full-service

Comments

With image acquisition and analysis system (Leica),

Equipment

High-throughput capabilities with GloMax®-Multi+Microplate Multimode Reader

Service type: self-service or full-service?

Full-service

Comments

(Promega) accepting 6, 12, 24, 48, 96 and 384-well plates, and equipped with luminometer, fluorescence, and visible/UV absorbance modules and dual injector system for 6 to 96-well plate formats. http://worldwide.promega.com/products/pm/fluorometers-luminometers-multimode-readers/glomax-multi-plus-instinct/

Equipment

xCELLigence RTCA SP (ACEA Biosciences)

Service type: self-service or full-service?

Full-service

Comments

For real-time label free impedence-based cell analysis in 96 well format. http://www.aceabio.com/product_info.aspx?id=178

Equipment

Multidrop Combi Reagent Dispenser (Thermo Scientific)

Service type: self-service or full-service?

Full-service

Comments

Dispensing volumes of 0.5 to 2500ul, in 6 to 1536 well plate formats. http://www.thermoscientific.com/en/product/multidrop-combi-reagent-dispenser.html

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