Services Centres
Flow Cytometry Unit -
Center for Neuroscience and Cell Biology
The Flow Cytometry Unit provides technical support on flow cytometry. Currently, it is equipped with a Becton Dickinson FACSCalibur cell analyser and a Partec CyFlow Space cell sorter. The cytometry unit provides scientific and technical support for those wishing to use flow cytometry in their studies and is available to all CNC researchers, as well for those external to the CNC, after prior arrangement.
The Flow Cytometry Unit provides technical support on flow cytometry. Currently, it is equipped with a Becton Dickinson FACSCalibur cell analyser and a Partec CyFlow Space cell sorter. The cytometry unit provides scientific and technical support for those wishing to use flow cytometry in their studies and is available to all CNC researchers, as well for those external to the CNC, after prior arrangement.
Advanced Flow Cytometry Unit -
IBMC - Institute for Molecular and Cell Biology
The AFCU provides investigators with equipment for acquisition and analysis of flow cytometric data and for cell sorting from single cell suspension using fluorescence. Furthermore, the AFCU gives training and consulting for researchers that intend to use flow cytometry in their projects; and helps analyzing and interpreting data. A number of applications, including the multicolour analysis of cell phenotype, gene expression, membrane potential, cell cycle, among others, can be performed.
The AFCU provides investigators with equipment for acquisition and analysis of flow cytometric data and for cell sorting from single cell suspension using fluorescence. Furthermore, the AFCU gives training and consulting for researchers that intend to use flow cytometry in their projects; and helps analyzing and interpreting data. A number of applications, including the multicolour analysis of cell phenotype, gene expression, membrane potential, cell cycle, among others, can be performed.
Flow Cytometry Shared Facility (FlowSF) -
Life and Health Science Research Institute (ICVS)
The FlowSF is equipped to high standards for cell analysis and enrichment by flow cytometry. Available equipment includes a LSRII (prepared to simultaneously read 8 different colors), a FACS Calibur and a AutoMACS Cell separator. The facility is prepared to analyze animal and human cells; the use of other cell types should be proposed to the FlowSF and optimized by the user. New users need specific training and previous approval before independently using the facilities.
The FlowSF is equipped to high standards for cell analysis and enrichment by flow cytometry. Available equipment includes a LSRII (prepared to simultaneously read 8 different colors), a FACS Calibur and a AutoMACS Cell separator. The facility is prepared to analyze animal and human cells; the use of other cell types should be proposed to the FlowSF and optimized by the user. New users need specific training and previous approval before independently using the facilities.
CYTOMETRY LABORATORY -
Faculty of Health Sciences, University of Beira Interior
This laboratory (24 m2) is equipped with a flow cytometer FACScalibur (4 colors), and a high-speed analyzer CyAn (Beckam Coulter) that can measure up to 9 colors. These instruments allow us to analyze biological cells, beads or other microscopic particles. With their multi-color capabilities, a wide range of flow cytometric research and clinical demands can be performed, such as immunophenotyping and cell viability analysis. Additionally, there is an AutoMACS cell separator (BD).
This laboratory (24 m2) is equipped with a flow cytometer FACScalibur (4 colors), and a high-speed analyzer CyAn (Beckam Coulter) that can measure up to 9 colors. These instruments allow us to analyze biological cells, beads or other microscopic particles. With their multi-color capabilities, a wide range of flow cytometric research and clinical demands can be performed, such as immunophenotyping and cell viability analysis. Additionally, there is an AutoMACS cell separator (BD).
GMP Manufacturing - Cells and Cell based Products -
Stemmatters, Biotecnologia e Medicina Regenerativa SA
Stemmatters provides cGMP services regarding tissue processing, manufacturing and banking (cells and cell based products).The facility comprises 250 m2 of qualified controlled environment rooms (CERs) and material pass-boxes. Our quality management system ensures that our products are consistently produced and controlled according to strict quality standards (EU-GMP Guide/ICH Q7 and ISO 9001:2008).We work with specialized distribution networks to assure high quality according to GMP guidelines.
Stemmatters provides cGMP services regarding tissue processing, manufacturing and banking (cells and cell based products).The facility comprises 250 m2 of qualified controlled environment rooms (CERs) and material pass-boxes. Our quality management system ensures that our products are consistently produced and controlled according to strict quality standards (EU-GMP Guide/ICH Q7 and ISO 9001:2008).We work with specialized distribution networks to assure high quality according to GMP guidelines.
Cell Biology -
iMed.ULisboa/ FFULisboa Research Institute for Medicines/ Faculdade de Farmácia, Universidade de Lisboa
This facility is dedicated to the analysis of gene expression at DNA, RNA and protein level, routinely performing a multitude of biochemical and molecular biology techniques using standard equipment for electrophoresis, western blot, northern bot, as well absorbance plate reader, and gel, membrane and X-ray film imaging (Chemidoc MP and XRS; Bio-Rad).
This facility is dedicated to the analysis of gene expression at DNA, RNA and protein level, routinely performing a multitude of biochemical and molecular biology techniques using standard equipment for electrophoresis, western blot, northern bot, as well absorbance plate reader, and gel, membrane and X-ray film imaging (Chemidoc MP and XRS; Bio-Rad).
Cell Function -
iMed.ULisboa/ FFULisboa Research Institute for Medicines/ Faculdade de Farmácia, Universidade de Lisboa
The facility is tailored for biological evaluation of cell function, routinely determining cytotoxic and cytoprotective activities of synthetic and natural compounds, in multiple cell models, including immortalized and primary cultures and stem cells, with implemented capability of cell function assays up to 96 and 384-well format.
The facility is tailored for biological evaluation of cell function, routinely determining cytotoxic and cytoprotective activities of synthetic and natural compounds, in multiple cell models, including immortalized and primary cultures and stem cells, with implemented capability of cell function assays up to 96 and 384-well format.
Biological and Chemical Analysis Facility -
UCIBIO, Universidade Nova de Lisboa, Universidade do Porto
The BioLab is a Biological and Chemical Analysis Facility at FCT-NOVA. This facility is devoted to the comprehension of cellular and molecular mechanisms, from the in vitro characterization of molecular interactions to the in vivo validation in model animals. The BioLab facility gathers in a single unit a unique set of state-of-the-art biophysical and biological technologies. For a complete characterization of your biological/chemical system please contact us biolab@campus.fct.unl.pt.
The BioLab is a Biological and Chemical Analysis Facility at FCT-NOVA. This facility is devoted to the comprehension of cellular and molecular mechanisms, from the in vitro characterization of molecular interactions to the in vivo validation in model animals. The BioLab facility gathers in a single unit a unique set of state-of-the-art biophysical and biological technologies. For a complete characterization of your biological/chemical system please contact us biolab@campus.fct.unl.pt.
Rodon Biologics -
Rodon Biologics
Rodon Biologics is a service provider company with more than 15 years experience in protein recombinant production andprocess development. Rodon is a spin-off of Biotecnol, a company operating in Portugal since 1996 dedicated to biological development. Rodon services include: protein production, analytical characterization, cell-based assays, cell line and process development. Rodon is customer-centric and guarantees that the projects are executed under a quality system.
Rodon Biologics is a service provider company with more than 15 years experience in protein recombinant production andprocess development. Rodon is a spin-off of Biotecnol, a company operating in Portugal since 1996 dedicated to biological development. Rodon services include: protein production, analytical characterization, cell-based assays, cell line and process development. Rodon is customer-centric and guarantees that the projects are executed under a quality system.
