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Service Centre

Mass Spectrometry Unit

Center for Neuroscience and Cell Biology

Service Centre category(ies)

Proteomics / Peptide Synthesis

Description of the Service Centre

The Unit is specialized in the identification and quantification of proteins from complex samples, identification of post-translational modifications, and identification and quantification of metabolites. It is mainly focused on running R&D projects from both partners, as well as other collaborators (from CNC, Biocant, other R&D institutes and private companies).

Service Centre web address

http://www1.biocant.pt/MassSpectrometry_at_CNC/index.htm

Contact person's name

Bruno Manadas

Contact person's e-mail

bmanadas@gmail.com

Contact person's telephone

351913510346

Last edited on

2014-10-05 13:29:38

Services

Service

targeted metabolite qantification

Available for external use?

Yes

Comments

The unit is specialized in quantification of drugs and small metabolites from different biological matrices

Service

Interactomics

Available for external use?

Yes

Comments

We perform large scale interactomics approaches with protein identificatino from pull-down or immunoprecipitation assays. The core facility should be contacted before these assays are performed in order to ensure proper sample handling for mass spectromet

Service

Protein identification and post-translational modifications identification

Available for external use?

Yes

Comments

The unit routinely performs protein identification as well as identification of post-translational modification with major emphasis on phosphorylation. Targeted analysis also includes identification of enzyme specificity and quantification of enzymatic ac

Equipments

Equipment

4000 QTRAP with the Ultimate 3000 multidimensional liquid chromatograph

Service type: self-service or full-service?

Full-service

Comments

Triple quadropole and ion-trap for targeted quantification of small molecules

Equipment

2D-nanoLC Ultra (Eksigent) coupled to the 5600 Triple TOF (ABsciex)

Service type: self-service or full-service?

Full-service

Comments

High resolution mass spectrometer with SWATH quantitative capability for protein identification and quantification, along with metabolite identification and quantification

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