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Service Centre

CYTOMETRY LABORATORY

Faculty of Health Sciences, University of Beira Interior

Service Centre category(ies)

Cell Analysis / Flow Cytometry

Description of the Service Centre

This laboratory (24 m2) is equipped with a flow cytometer FACScalibur (4 colors), and a high-speed analyzer CyAn (Beckam Coulter) that can measure up to 9 colors. These instruments allow us to analyze biological cells, beads or other microscopic particles. With their multi-color capabilities, a wide range of flow cytometric research and clinical demands can be performed, such as immunophenotyping and cell viability analysis. Additionally, there is an AutoMACS cell separator (BD).

Service Centre web address

Service Centre web address

Contact person's name

Mafalda Fonseca

Contact person's e-mail

mfonseca@fcsaude.ubi.pt

Contact person's telephone

00351 275329056

Last edited on

2013-09-20 11:43:32

Services

Service

Cell analysis

Available for external use?

Yes

Comments

The available equipments allow the analysis of fluorescence on cells/particles either in the form of either antibodies or dyes, and also their morphological characteristics. The service is available for external trained users. The cytometry laboratory has

Service

Cell analysis

Available for external use?

Yes

Comments

The available equipments allow the analysis of fluorescence on cells/particles either in the form of either antibodies or dyes, and also their morphological characteristics. The service is available for external trained users. The cytometry laboratory has

Equipments

Equipment

AutoMACS cell separator (BD)

Service type: self-service or full-service?

Self-service

Comments

The AutoMACS is an automated bench-top magnetic sorting system capable of sorting millions of cells per second for positive selection as well as depletion.

Equipment

FACScalibur (Becton Dickinson, BD)

Service type: self-service or full-service?

Self-service

Comments

Provides a user-friendly multicolor analysis, equipped with 488nm and 635nm lasers, capable of detecting up to four fluorescent signals and 2 morphological characteristics (FSC, SSC).

Equipment

CyAn (Beckam Coulter)

Service type: self-service or full-service?

Self-service

Comments

Cyan uses multiple laser excitation sources to analyze biological cells, beads or other microscopic particles. It utilizes high performance digitization with pulse processing speed up to 70,000 events per second. It is equipped with three excitation lasers: 405nm, 488nm, 642nm, and can measure up to 11 standard parameters: 9 colors (2 violet, 5 blue, and 2 red ), as well as FCS and SSC.

Equipment

AutoMACS cell separator (BD)

Service type: self-service or full-service?

Self-service

Comments

The AutoMACS is an automated bench-top magnetic sorting system capable of sorting millions of cells per second for positive selection as well as depletion.

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