Service Centre category(ies)
Biochemical / Chemical / Structural Analysis
Molecular fractionation, isolation, purification and production
Proteomics / Peptide Synthesis
Spectrometry / Crystallography
Description of the Service Centre
The Protein Production and Purification Unit (UP3) provides access to state-of-the-art equipment for the expression, purification and biochemical/biophysical analysis of recombinant proteins. This facility provides both scientific and technical expertise, actively participating in the establishment of interdisciplinary research and networking activities involving internal and external research groups.
Service Centre web address
http://www.ibmc.up.pt/research/research-facilities/protein-production-and-purification-unit
Contact person's name
Frederico Silva
Contact person's e-mail
ffsilva@ibmc.up.pt
Contact person's telephone
351226074900
Service
Recombinant protein production
Available for external use?
Yes
Comments
Heterologous expression of proteins in prokaryotic and eukaryotic systems, mainly E. coli and Insect cells through baculovirus vectors. The UP3 lab is fully equipped to meet the needs of every step of the protein production process, such as cell growth,
Service
Protein Purification
Available for external use?
Yes
Comments
The UP3 lab is fully equipped to meet the needs of every step of the protein purification process, such as cell harvesting and lysis and protein purification. To that end the lab provides access to: - an high capacity centrifuge - ultrasonic and high pr
Service
Macromolecule Analysis
Available for external use?
Yes
Comments
The Protein Production and Purification Unit also provides access to state-of-art physical biochemistry analytic instrumentation, including: - double beam UV-Vis spectrophotometer with thermostatic control and 6 cell positioner - high end, photon counti
Service
Biomolecular interaction analysis
Available for external use?
Yes
Comments
The Protein Production and Purification Unit provides access to state-of-art physical biochemistry analytic instrumentation enabling the characterization of binding events , including: - high end, photon counting spectrofluorimeter for ultrasensitive flu
Service
Chromatography
Available for external use?
Yes
Comments
The unit has an extensive expertise in analytical and preparative chromatography of Biomacromolecules. The Unit is equiped with cromatography equipment (5) at 4ºC and at room temperature, including two FPLCs, two low pressure systems and an HPLC, allowin