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Service Centre

Protein Production and Purification Unit

IBMC - Institute for Molecular and Cell Biology

Service Centre category(ies)

Biochemical / Chemical / Structural Analysis
Molecular fractionation, isolation, purification and production
Proteomics / Peptide Synthesis
Spectrometry / Crystallography

Description of the Service Centre

The Protein Production and Purification Unit (UP3) provides access to state-of-the-art equipment for the expression, purification and biochemical/biophysical analysis of recombinant proteins. This facility provides both scientific and technical expertise, actively participating in the establishment of interdisciplinary research and networking activities involving internal and external research groups.

Service Centre web address

http://www.ibmc.up.pt/research/research-facilities/protein-production-and-purification-unit

Contact person's name

Frederico Silva

Contact person's e-mail

ffsilva@ibmc.up.pt

Contact person's telephone

351226074900

Services

Service

Recombinant protein production

Available for external use?

Yes

Comments

Heterologous expression of proteins in prokaryotic and eukaryotic systems, mainly E. coli and Insect cells through baculovirus vectors. The UP3 lab is fully equipped to meet the needs of every step of the protein production process, such as cell growth,

Service

Protein Purification

Available for external use?

Yes

Comments

The UP3 lab is fully equipped to meet the needs of every step of the protein purification process, such as cell harvesting and lysis and protein purification. To that end the lab provides access to: - an high capacity centrifuge - ultrasonic and high pr

Service

Macromolecule Analysis

Available for external use?

Yes

Comments

The Protein Production and Purification Unit also provides access to state-of-art physical biochemistry analytic instrumentation, including: - double beam UV-Vis spectrophotometer with thermostatic control and 6 cell positioner - high end, photon counti

Service

Biomolecular interaction analysis

Available for external use?

Yes

Comments

The Protein Production and Purification Unit provides access to state-of-art physical biochemistry analytic instrumentation enabling the characterization of binding events , including: - high end, photon counting spectrofluorimeter for ultrasensitive flu

Service

Chromatography

Available for external use?

Yes

Comments

The unit has an extensive expertise in analytical and preparative chromatography of Biomacromolecules. The Unit is equiped with cromatography equipment (5) at 4ºC and at room temperature, including two FPLCs, two low pressure systems and an HPLC, allowin

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